Below is the sequence of the HEXA gene with the underlined portion the 1278TATC allele that recessively caused Tay-Sachs disease. Answer the following questions about this sequence: 5’ 3’

3’ 5’

a. Identify all the PAM sites by clearly marking them in the sequence above.
b. Pick one that is appropriate for the goal of removing the 4 extra TATC nucleotides. There are multiple good answers and you should only choose one.
c. Design a 20bp sgRNA spacer sequence that will make an appropriate cut. There are multiple good answers, and you should only choose one. Be sure to label 5’ and 3’ and the side that would attach to the scaffold sequence.
d. Would you use an HDR template or NHEJ to consistently make the correct edit? Explain

Apart of the periodic table is shown. a portion of the sixteenth and seventeenth columns of the periodic table is shown. column 16 reads eight oxygen, 16 sulfur, and 34 selenium. column 17 reads nine flourine, 17 chlorine, and 35 bromine. which of the following elements is less reactive than the others? bromine (br) oxygen (o) selenium (se) sulfur (s)

On a floor there are several 1-4 mm elongated drops leading away from a body lying in a pool of blood. the the forward wall there is a sigzag pattern and on the walls to the right side there is a linear patter

Approximately 90% of all cases of polycystic kidney disease are inherited in an autosomal dominant fashion. the disease is typically related to mutations to genes (pkd1 and pkd2). pkd genes can be found on chromosome 16. the disease occurs in approximately 1: 800 to 1: 1000 people. here is a hypothetical example. the dominant allele d will cause the development of pkd. the recessive allele is known as d. what genotypes would correspond to suffering from pkd? dd, dd what genotypes would correspond to being normal (not suffering from pkd)? dd consider that 1: 1,000 people have pkd in your population of 325million. consider that pkd is inherited in an autosomal dominant fashion. show you math for all of the following questions: what is the genotypic frequency for dd? what is the genotypic frequency for dd? what is the genotypic frequency for dd? what is p? what is q? how many people in your population would have pkd?