Biology

Biology, 31.08.2019 13:00, baeethtsadia

What would be the optimum wavelength for measuring the absorbance versus concentration of a series of fd& c blue 1 dye solutions?

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Physics, 25.06.2019 10:40, ella3714

Access link #1 and read the section about light intensity (i0 and i), transmittance (t) and absorbance (a). as described in question 1, an absorbance spectrum is a graph that illustrates the correlation between wavelength and absorbance. the wavelength of maximum absorbance, λmax, is where the absorbance is the greatest in the absorbance spectrum. access the simulation of link #2 to (1) create an absorbance spectrum between 200 nm and 700 nm with 10 to 15 data points, and (2) determine the wavelength of maximum absorbance to within 5 nm. the simulation will you understand the use and function of a uv-vis spectrophotometer in our lab. approach (link #2): run simulation to obtain the transmitted light intensity at various wavelengths (between 200 nm and 700 nm, 10 to 15 data points). each simulation should be run long enough to detect at least 10000 photons. for each wavelength, calculate the transmittance of the solution (light intensity of blank is 3300 photons/second; t = i÷ i0). calculate the absorbance of the solution using transmittance at each wavelength (a = −log10( create the absorbance spectrum by plotting absorbance (y-axis) versus wavelength (x-axis). determine λmax by locating the wavelength at the maximum absorbance.

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Chemistry, 10.10.2019 05:30, Rboltonbcs

We work with solutions of the dye coomasie blue in water. these solutions have a blue color and absorb light of wavelength 470nm. our goal is to get an equation relating the concentration of the dye and the absorbance of its solution. given below are the absorbances measured for different concentrations of coomasie blue concentration (mm/l) absorbance (no units) 8.243606354 60.9124698 272.9907502 1223.459661 1. using rectanguladog log/semi-og graphs, find the equation relating absorbance and dye concentration (10 points) what are the values and units of the constants in the equation (10 points) . two collaborators are interested in using the concentration vs. absorbance equation you developed but they require it to be rewritten for dye concentrations that are measured in midmand in g/ml. rewrite the equation to accept concentration in each of these two units give the values and units for the constants in the two new equations. note that the molecular weight of coomasie blue is 853 grams/mole

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Chemistry, 18.11.2019 20:31, Thunderalesis7855

Astudent uses visible spectrophotometry to determine the concentration of cocl2(aq) in a sample solution. first the student prepares a set of cocl2(aq) solutions of known concentration. then the student uses a spectrophotometer to determine the absorbance of each of the standard solutions at a wavelength of 510nm and constructs a standard curve. finally, the student determines the absorbance of the sample of unknown concentration. a wavelength of 510nm corresponds to an approximate frequency of 6×1014s−1. what is the approximate energy of one photon of this light? 9×1047j.

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