Biology
Biology, 03.04.2021 04:50, emblemhacks

Blue-white screening is a technique for the identification of___. It relies on the ability ofto cleave lactose. Blue-white tests take advantage of a molecule calledwhich is similar to lactose in that it is cleaved by B-galactosidase. When cleaved, the(same as previous) turns . If uncleaved, meaning a non- function B-gal gene, the X-gal remains. Therefore, abacterial colony means the B-galactosidase gene is not-functional, and therefore therea recombinant gene inserted into the vector. You’ve inserted a gene for pesticide resistance into your vector. To see if it was successful, you decide to conduct a blue-white screening test. But, uh-oh you are out of X-gal! You do notice some green Slime-gal sitting on the shelf and recall that this molecule works similarly to X-gal, but instead of turning blue when cleaved, it turns green when cleaved. You carry out the test, and find that all of your colonies are green! Was your insertion of the pesticide gene successful?

answer
Answers: 2

Other questions on the subject: Biology

image
Biology, 21.06.2019 19:50, karinapenn8259
Which statements correspond to cellular respiration? co2 diffuses passively into the cell. co2 diffuses passively out of the cell. co2 must be pumped out of the cell. o2 diffuses passively when produced inside the cell. o2 diffuses passively when converted to co2. o2 is pumped in and forces co2 out.
Answers: 3
image
Biology, 22.06.2019 09:40, QueenMiah16
Explain how paleontologists use trilobite fossils as index fossils for various geologic time periods.
Answers: 1
image
Biology, 22.06.2019 13:50, wyattgrubb00
2points what do bacteria have in common with the cells of other living organisms?
Answers: 2
image
Biology, 22.06.2019 15:00, Gunn2368
Which of the following must be true for a fossil organism to be useful as an index fossil? a. the fossil organism must be widespread geographically. b. the fossil organism must be abundant. c. the fossil organism must be limited to a short span of geologic time. d. all of the above.
Answers: 1
Do you know the correct answer?
Blue-white screening is a technique for the identification of___. It relies on the ability ofto clea...

Questions in other subjects:

Konu
Chemistry, 06.11.2020 17:50