Biology, 26.07.2020 14:01, robertss403
You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not more than 2 pages (using 1.5 line space of Arial or Times New Roman fonts) provide answers for the following questions? 1) Which tube from the three is the most appropriate to use and why? 2) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which has a 5¢-recognition site and a 3¢- HindIII in the MCS? 3) How can you guarantee a high expression of your protein in any expression vector?
Answers: 2
Biology, 21.06.2019 19:50, bgallman153p71edg
Which of the following statements about c4 carbon fixation is not true? a c4 carbon fixation is an adaptation for plants exposed to high light intensityb. c4 carbon fixation occurs in more plants than c3 carbon fixation. c c4 carbon fixation is more common in areas of high temperatures than c3 carbon fixation. d. c4 carbon fixation occurs in the inner cells of a leaf rather than the entire leaf.
Answers: 3
Biology, 22.06.2019 07:30, jaylabazemore
What is one way intensive agriculture can contribute to climate change? a. tree loss to agriculture increases earth's albedo b. livestock manure absorbs greenhouse gases c. large herds of livestock release greenhouse gases d. fewer trees are available to replenish petroleum stores appex
Answers: 2
Biology, 22.06.2019 07:50, jadejordan8888
45 points how are people today being protected from tsunamis? earthquake data is analyzed to detemrine if a tsunami is likely, and if so, warnings are sent out. areas in tsunami risk zones are no longer heavily populated. radar is used to measure wave heights regularly. meteorologists study the winds to determine if wave heights will be large.
Answers: 1
You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin...
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