It can be desirable to produce eukaryotic proteins in prokaryotes such as E. coli. To do this several DNA sequences must be joined or cloned together. Place the labels in the appropriate position to allow for the expression of the insulin protein in a prokaryotic cell. cDNA is DNA that is synthesized from the mature mRNA from eukaryotic cells using the enzyme reverse transcriptase.

5' end 3' end

1. Poly A signal sequence
2. Genomic clone of insulin
3. Rho terminator
4. -95 CAT box
5. -10 TATA
6. -25 TATA box
7. cDNA of insulin
8. -35 Sequence

The position of these sequences is:

5' end

TATA box

Genomic clone of insulin

Rho terminator

Poly A signal sequence

3' end

Explanation:

The TATA box is a DNA sequence localized in promoter gene regions in organisms ranging from bacteria to eukaryotes

Genomic clone of insulin whose transcription to produce the protein of interest

Rho terminator is a pause sequence involved in the termination of transcription in prokaryotes

Polyadenylation is the addition of a poly Adenine (A) tail to a messenger RNA

"a.) well–developed vascular system."

the areas around the equator