Suppose you have identified a mutant strain of yeast that cannot grow without leucine added to the growth medium. you know that the leu- phenotype results from a single mutation, but you do not know where in the yeast genome the mutation occurred. which of the following strategies would you use to clone the wild-type gene corresponding to the mutant allele? only one answer correct will be considered correct.(a) pcr amplification of the gene using flanking primers(b) screening of a yeast-cdna expression library using an antibody probe(c) complementation of the leu- phenotype with a yeast genomic plasmid library(d) hybridization of a dna probe to a southern blot(e) hybridization of a dna probe to a northern blot(f) screening of a yeast-genomic λ phage library using a dna probe

Where does all the water go? according to the environmental protection agency (epa), in a typical wetland environment, 39% of the water is outflow; 46% is seepage; 7% evaporates; and 8% remains as water volume in the ecosystem (reference: united states environmental protection agency case studies report 832-r-93-005). chloride compounds as residuals from residential areas are a problem for wetlands. suppose that in a particular wetland environment the following concentrations (mg/l) of chloride compounds were found: outflow, 60.4; seepage, 73.7; remaining due to evaporation, 26.4; in the water volume, 46.8. (a) compute the weighted average of chlorine compound concentration (mg/l) for this ecological system. (round your answer to one decimal place.) mg/l (b) suppose the epa has established an average chlorine compound concentration target of no more than 58 mg/l. does this wetlands system meet the target standard for chlorine compound concentration? yes. the average chlorine compound concentration (mg/l) is too high. yes. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is lower than the target. no. the average chlorine compound concentration (mg/l) is too high.