Which of the following correctly describes a simple stain?
a. a student applies crystal violet to a smear, rinses, then observes.
b. a student gram stains a smear, then observes.
c. all are correct.
d. a student observes an unstained smear.

a.A student applies crystal violet to a smear, rinses, then observes.

Explanation:

A simple stain is a straightforward staining process that involves no more than one stain. The range of options includes Gram safranin, methylene blue, and Gram crystal violet. These stains are implemented to stain the major part of bacteria by giving off a hydroxide ion or taking in a hydrogen ion, leaving itself with a positive charge. As bacteria have a negative charge, these stains stick to the surface of their cells.

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