What can be correctly concluded from this experiment? A sealed bottle containing bacterial cells and nutrients needed for cell growth is exposed to intense heat. It is then cooled. The bottle is not opened. Once the bottle has cooled, scientists note that even after several weeks, no bacteria growth occurs in the bottle.

1. New cells can only form from existing, living cells.
2. New cells can only form from nutrients if energy is provided.
3. New cells can only form in the presence of nutrients.
4. New cells can form from nonliving materials.