What is the role of ligase in inserting a restriction fragment into a dna vector?

Find equation of line with slope equals 8 and passing through (6,53)

Aman is 6 feet 3 inches tall. the top of his shadow touches a fire hydrant that is 13 feet 6 inches away. what is the angle of elevation from the base of the fire hydrant to the top of the man's head?

The ivring berlin learning center would like a possible net profit of $650,000 on its new resource manual it sells to schools. the fixed costs for producing the manual are $780,000 and the variable cost per package is $19.85. if the estimated unit sales are 1,250,000 units, what selling price unit should the ivring berlin learning center try?