When is subtraction always the first operation done in an expression? Never
When it follows addition in an expression.
When it is the first operation in an expression, from left to right.
When it is in parentheses in an expression.

The biologist has a second culture to examine. she knows that the population of the culture doubles every 15 minutes. after 1 hour and 15 minutes, her assistant found that 80,000 bacteria were present. a) what was the size of the initial population? b) predict the size of the culture at t=3 hours. what was the size of the population at 40 minutes? explain and justify your answers. c) create a graph of the population as a function of time. find an equation that can be used to predict the size of the population at any time t. d) examine the rate at which the bacteria culture is growing. how fast is the culture growing after 1 hour? after 1.5 hours? after 2 hours? use a time interval of h = 0.01 hours to estimate these rates. interpret these rates in terms of the context of the problem situation. how do these three rates compare?