How did the design of pasteur's flask him successfully refute the hypothesis of spontaneous generation?

answer; /// i believe that the correct answer is (d)the huang he river valley;

frederick william of brandenburg-prussia trained his son, frederick, to be a hardy soldier. but frederick disliked rifle shooting and horseback riding. unlike his father, he was interested in french literature. he enjoyed poetry and music and, like some others of the enlightenment, he scoffed at religion. his father thought him soft, frivolous and unmindful of his duties. and when frederick refused to put his signature on a right to succession, for a few moments his father strangled him.