the correct answer is native americans
congress passed the 1785 ordinance, which took precedence over the ordinance of 1784. this ordinance required that the new lands be measured before being distributed or sold. the lands would be measured in sections called six-square-mile townships. each of these townships would be subdivided into thirty-six sections of one square mile each, which is the equivalent of 640 acres. the government could auction lots of one acre, or even a smaller subdivision, as long as they were sold at no less than $ 1 per acre. the ordinance also specified that in each township one of the lots of one square mile had to be dedicated to maintain public schools.
the first ordinance drafted by thomas jefferson, divided the territory into autonomous districts and fixed a minimum population to achieve the status of state. the final ordinance, drafted in part by rufus king, established the extension and prices of land grants, provided public lands for schools, banned slavery and guaranteed civil liberties; in addition, it established the principle according to which new states were admitted under equal conditions with respect to the original 13.
because of this we can say that the most affected were the antivos americans, since they were not taken into account when measuring the lands and distributing them. also they were the very owners of the territory.