Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. ch09 10/11/01 5:33 PM Page 281 a. Determine the culture volume at t = 10 h. b. Determine the concentration of glucose at t = 10 h at quasi-steady state. c. Determine the concentration and total amount of cells at quasi-steady state when t = 10 h. d. If qP = 0.05 g product/g cells h and P0 = 0.1 g/l, determine the product concentration in the vessel at t = 10 h.