8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

Ahouse has the following electrical appliance usage (1) single 40w lamp used for 4 hours per day (2) single 60w fan used for 12 hours per day (3) single 200w refrigerator that runs 24 hours per day with compressor run 12 hours and off 12 hours find the solar power inverter size in watt with correction factor of 1.25.

A3-mm-thick panel of aluminum alloy (k 177 w/m-k, c 875 j/kg-k and ? = 2770 kg/m) is finished on both sides with an epoxy coating that must be cured at or above t,-150°c for at least 5 min. the production line for the curing operation involves two steps: (1) heating in a large oven with air at ts,0-175°c and a convection coefficient of h, 40 w/m2. k, and (2) cooling in a large chamber with air at 25°c and a con- vection coefficient of he 10 w/m2.k. the heating portion of the process is conducted over a time interval te which exceeds the ime required to reach 150°c by 5 min (h = r + 300 s). the coating has an emissivity of ? = 0.8, and the temperatures of the oven and chamber walls are 175 and 25°c, respectively. if the panel is placed in the oven at an initial temperature of 25°c and removed from the chamber at a safe-to-touch tempera ture of 37°c, what is the total elapsed time for the two-step curing operation?

Air inially occupying a volume of 1 m2 at 100 kpa, 27 c undergoes three internally reversible processes in series. process 1-2 compression to 500 kpa during which pv constant process 2-3 adiabatic expanslon to 100 kpa process 3-1: constant-pressure expansion to 100 kpa (a) calculate the change of entropy for each of the three processes. (b) calculate the heat and work involved in each process. (c) is this cycle a power cycle or refrigeration cycle?