Engineering, 11.12.2019 22:31, fili20

Radioactive-labeling experiments can yield estimates of how much glucose-6-phosphate is metabolized by the pentose phosphate pathway and how much is metabolized by the combined action of glycolysis and the citric acid cycle. suppose that you have homogenate samples of two different tissues as well as two radioactively labeled glucose samples, one with glucose labeled with 14c at c-1 and the other with glucose labeled with 14c at c-6. a. design an experiment that would enable you to determine the relative activity of the aerobic metabolism of glucose compared with metabolism by the pentose phosphate pathway in these two tissues. (5 points) b. predict the expected outcomes. (5 points) c. provide the rationale for your experimental design. (5 points)

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Radioactive-labeling experiments can yield estimates of how much glucose-6-phosphate is metabolized...