Calculate the mass of chloroform actually injected onto the GC column when you injected pure chloroform at the beginning. To do this you will need the volume injected, the split ratio, and the density of the compound. The split ratio is the volume injected that does not go to the column (and is vented instead) for every volume that does go onto the column. For example, if the split ratio is 100:1, then only 1/101 of the amount injected from the syringe actually goes onto the column (one part to GC, 100 parts vented).Express the answer in micrograms. There was 1 \mu L of chloroform injected and the split ratio is 1:100. Therefore, only one part of the chloroform goes into the column, the rest 99 parts is vented. Density of chloroform = 1.49 g/cm^3