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You have obtained the following data from a series of enzyme kinetics experiments. Given that the enzyme concentration used in the experiments is 10
nM, estimate the kcat for this enzyme? Your answer must be in the units of s-1, rounded off to the nearest whole number (for example, 34).
[SI'M
Vo M/sec
0
0
15
0.21
30
0.33
40
0.39
100
0.55
300
0.68
1000
0.74
5000
0.76