Enolase, an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate: Group of answer choices demonstrates a base-catalysis step, where a proton is removed from the substrate. demonstrates an acid-catalysis step, where a proton is added to the substrate. requires two Mg2 cofactors and as such demonstrates metal-ion catalysis. stabilizes the intermediate and transition

Requires two Mg²⁺ cofactors and as such demonstrates metal-ion catalysis

Explanation:

Electrostatic catalysis or metal ion catalysis is a catalytic mechanism that makes use of metalloenzymes, such as enolase along with a metal ion which is bound tightly, including, Mo⁶⁺, Ni³⁺, Co³⁺, Mn²⁺, Zn²⁺, Cu²⁺, and Fe²⁺, to undertake a catalysis

For maximal activity, enolase requires the presence of 2 equivalent metal ions in each site which is active

Therefore, the correct option is; requires two Mg²⁺ cofactors and as such demonstrates metal-ion catalysis

answer: - 3.) the oxidation state of nitrogen in no changes from +2 to 0, and the oxidation state of carbon in co changes from +2 to +4 as the reaction proceeds.

solution: - as per the rules, oxidation number of oxygen in its compounds is -2. the sum of oxidation numbers of all the elements of a molecule or compound is zero.

also, the oxidation number in elemental form is zero.

in no, the oxidation number of o is -2 so the oxidation number of n is +2 so that the sum is zero.

similarly, the oxidation number of c in co is +2 and o is -2.

on product side the oxidation number of n is 0 as it is in the elemental form() .

oxidation number of c in carbon dioxide is +4 as there are two oxygen in it and we know that each oxygen is -2.

so, oxidation number of n is changing from +2 to 0 and the oxidation number of c is changing from +2 to +4.

since nitrogen is reduced and hence no is acting as an oxidizing agent. carbon is oxidized and so co is acting as a reducing agent.

so, the right choice is number 3.)the oxidation state of nitrogen in no changes from +2 to 0, and the oxidation state of carbon in co changes from +2 to +4 as the reaction proceeds.