A TLC plate showed 2 spots with Rf values of 0.25 and 0.26. The plate was removed from the developing chamber, the residual solvent was allowed to evaporate from the plate, and then the plate was returned to the developing chamber. What would you expect to see after the second development was complete

See explanation

Explanation:

TLC is a chromatographic method in which the solute is spotted on a plate and the plate is placed in an air tight chamber containing a solvent. The solvent is maintained below the level of the spot. The capillary movement of the solvent through the plate achieves the required separation.

If two spots have Rf values of 0.25 and 0.26 respectively and then the plate was removed from the developing chamber, subsequently, the residual solvent was allowed to evaporate from the plate, and then the plate was returned to the developing chamber.

It will be observed after the second development is complete that the new Rf values will be 0.50 and 0.52 respectively. It will just be as though the second chromatogram picked up from where the first chromatogram stopped.

the volume of fluid displaced would equal the volume of the gas. use a gas syringe - this is a laboratory instrument that can be used to withdraw a certain volume of gas from a closed system or to measure the volume of gas from a chemical reaction.