Chemistry, 29.05.2021 01:00, QueenThao8933
The free energy of ATP hydrolysis is used to maintain the concentration of Ca2 inside the cell to be constant. Calculate the number of moles of ATP required to pump out one mole of Ca2 when the cell is placed in a liquid culture containing 1 M CaCl2 at 25oC. The concentrations of ADP and Pi inside the cell are 250 mM and 10 mM, respectively, and that of ATP is 1.5 mM. The standard free energy of ATP hydrolysis at 25oC is O31.0kJ.
Answers: 1
![ATP\ +\ H_{2}O\ \rightleftharpoons \ ADP\ +\ P_{i}\\\\Q\ = \ \frac{[ADP][P_{i}]}{[ATP][H_{2}O]}\\\\ADP= 0.250 \ M\\\\P_i = 0.010 \ M\\\\ATP = 0.150 \ M\\\\H_2O = 55.55 \ M\\\\\Delta G \ =\ \Delta G^{o}\ +\ 2.303\ RT\ \log\ Q\\\\R = 8.314 \frac{J}{mol\ K}\\\\T = 298\ K\\\\](/tpl/images/1354/5228/25aff.png)
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