2.a calibration curve requires the preparation of a set of known concentrations of cv, which are usually prepared by dieting a stock solution whose concentration is known. describe how to prepare 10. ml of a 5, 10, 15, and 20 µm cv solution using a 25 µm cv stock solution.

In order to prepare 10 mL, 5 μM; 2 mL of the 25 μM stock solution will be taken and diluted with water up to 10 mL mark.

In order to prepare 10 mL, 10 μM; 4 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.

In order to prepare 10 mL, 15 μM; 6 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.

In order to prepare 10 mL, 20 μM; 8 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.

Explanation:

Using the dilution equation:

no of moles before dilution = no of moles after dilution.

Molarity x volume (initial)= Molarity x volume (final).

In order to prepare 10 mL, 5 μM from 25 μM solution,

Final molarity = 5 μM, final volume = 10 mL, initial molarity = 25 μM, initial volume = ?

25 x initial volume = 5 x 10

Initial volume = 50/25

                       = 2 mL

2 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.

In order to prepare 10 mL, 10 μM from 25 μM stock,

25 x initial volume = 10 x 10

Initial volume = 100/25 = 4 mL

4 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.

In order to prepare 10 mL, 15 μM from 25 μM stock,

25 x initial volume = 15 x 10

initial volume = 150/25 = 6 mL

6 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.

In order to prepare 10 mL, 20 μM from 25 μM stock,

25 x initial volume = 20 x 10

initial volume = 200/25 = 8 mL

8 mL of the 25 μM stock solution will be taken and diluted up to 10 mL mark.

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