The fluorescence of a CFP solution is quenched with a 30 mM solution of YFP, where the observed fluorescent lifetime is 0.5 s and the quenching rate is 43.0 M-1 s-1. Use the Stern-Volmer equation to find the ratio of non-quenched to quenched fluorescence, and then use the inverse of this ratio to calculate the percentage change in fluorescence upon quenching.

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definition

                    entropy is a measure of the randomness or the extent of disorder of a chemical process.

                      enthalpy is a measure of the heat change of a reaction occurring at a constant pressure.

measurement units:

                        entropy is measured in joule per kelvin (jk⁻¹)   whereas enthalpy is measured in joule per mole (jmol⁻¹)

requirements:

                            entropy has no requirements or limits, and its change is measured by the division between the heat change of the chemical process and the temperature.

                              in contrast, enthalpy can only be related to the heat change of the reaction under standard conditions.