Chemistry, 25.02.2021 18:50, alexisbrad3107
Tris is a molecule that can be used to prepare buffers for biochemical experiments. It exists in two forms: Tris (a base) and TrisH (an acid). The MW of Tris base is 121.14 g/mol; the MW of TrisH is 157.6 g/mol (the extra weight is due to the Cl- counterion that is present in the acid). The Ka of the acid is 8.32 X 10-9. Assume that you have TrisH in solid form (a powder), unlimited 1M HCl, 1 M NaOH and distilled water. How would you prepare 1 L of a 0.02 M Tris Buffer, pH?
Answers: 3
![$Ka = \frac{[\text{Tris}^- \times H_3O]}{\text{Tris}^+}$](/tpl/images/1147/5192/039c7.png)
, we have 
, one must have the 
concentration of the :![$\text{[OH}^-]=10^{-pOH} = 6.31 \times 10^{-7} \text{ moles of base}$](/tpl/images/1147/5192/2ecff.png)
gauge to 1000 mL with water.
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Tris is a molecule that can be used to prepare buffers for biochemical experiments. It exists in two...
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