The 10x SDS gel electrophoresis buffer contains 250mM Tris HCl, 1.92M Glycine, and 1% (w/v) SDS. Buffers are always used at 1x concentration in the lab (unless specified otherwise in the protocol), so you will have to dilute the 10x buffer to 1x before use. What is the concentration of Tris and SDS in the 1x buffer

25 mM Tris HCl and 0.1% w/v SDS

Explanation:

A 10X solution is ten times more concentrated than a 1X solution. The stock solution is generally more concentrated (10X) and for its use, a dilution is required. Thus, to prepare a buffer 1X from a 10X buffer, you have to perform a dilution in a factor of 10 (1 volume of 10X solution is taken and mixed with 9 volumes of water). In consequence, all the concentrations of the components are diluted 10 times. To calculate the final concentration of each component in the 1X solution, we simply divide the concentration into 10:

(250 mM Tris HCl)/10 = 25 mM Tris HCl

(1.92 M glycine)/10 = 0.192 M glycine

(1% w/v SDS)/10 = 0.1% w/v SDS

Therefore the final concentrations of Tris and SDS are 25 mM and 0.1% w/v, respectively.

explanation:

venn diagram is pictorial representation with of circles showing the relation between two finite sets or things. the intersection part of two circles conveys the relation present in between those sets or things.

here in the venn diagram of sand and salt two circles represent sand and salt. but the area falling under the intersection of these circle represents the mixture of sand and salt.

so, in a venn diagram the mixture will be placed in a middle that is in between the intersection of two circles.

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explanation:

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