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Why does DNA dissolve in water?

Why is salt added to solution of DNA and water?

How does ethanol help the precipitate form?

After a pellet is formed, is the DNA in the pellet or the supernate?

Explanation:

Because of this, DNA and RNA can easily dissolve in water. ... Adding ethanol helps the Na+ and PO3- ions come together, because ions travel easier in ethanol than water. The supernate is removed and new ethanol is added in the second washing. This removes any residual salt that remained on the pelleted DNA.

DNA is polar due to its highly charged phosphate backbone. Its polarity makes it water-soluble (water is polar) according to the principle "like dissolves like". ... This fact makes water a very good solvent for charged compounds like salts.

Your DNA's sugar phosphate backbone is charged. By adding salt, we help neutralize the DNA charge and make the molecule less hydrophilic, meaning it becomes less soluble in water. The salt also helps to remove proteins that are bound to the DNA and to keep the proteins dissolved in the water.

It is well known that Ethanol has a lower dielectric constant than water, making it to promote ionic bond formations between the Na+ (from the salt) and the PO3- (from the DNA backbone), further, causing the DNA to precipitate.

Explanation:

Ideally DNA needs to be precipitated with pellet and should not remain in supernatant. DNA is acidic in nature and therefore, needs optimum salt concentration in the buffer to be pelleted from a solution. At very low salt concentration or without salt DNA would remain in supernatant.

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