What is the benefit of substrate channeling? The PDH active site forms in the hydrophobic core of the complex instead of a surface‑exposed region. Reaction intermediates move to sequential active sites faster than the diffusion constant. Every intermediate or product made by the PDH complex enters the citric acid cycle as a substrate. The PDH complex sequesters excess substrate to use at later time. Intermediates of a multistep reaction sequence do not dissociate from the enzyme complex.

Intermediates of a multistep reaction sequence do not dissociate from the enzyme complex.

Explanation:

Substrate channeling is the process in which intermediates produced in an enzymatic reaction is transferred to another enzyme or active in a reaction pathway without the substrate having to dissociate from the enzymes active sites into the reaction medium or solution.

Benefits of substrate channeling include:

Substrate channeling is observed in the pyruvate dehydrogenase complex reaction which oxidizes pyruvate to acetyl-CoA.

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the ideal gas law equation is as follows

pv = nrt

where p - pressure

v - volume

n - number of moles

r - universal gas constant

t - temperature

so if the pressure, volume and temperature are already known

we are left with n and r

since r is the universal gas constant that has a known fixed value then r too is known

so we are left with 'n'

once we know temperature volume and pressure

we can find the number of moles of gas present using the ideal gas law equation

this is not my own answer, this is an answer for the same question by the user blahblahmali. i do not claim ownership of this account.