Which statement regarding regulation of the citric acid cycle is false? Ca2+ is involved in citric acid cycle regulation in vertebrate muscle. Dysfunction of the citric acid cycle (e. g., due to mutations in enzymes of the cycle) is often associated with different types of cancer. As [NADH]/[NAD+] increases, flux through the cycle increases. In addition to regulation of the PDH complex, regulation of the citric acid cycle occurs by regulation of the enzymes that catalyze the strongly exergonic steps.

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answer : one can design an experiment using a liquid and an additive that can be added to the liquid and observe the chemical changes in the reaction species.

one would need a beaker which is filled with a known amount of the liquid in which thermometer is placed to measure the temperature changes; after addition of the additive to the liquid solution one can note down the temperature change and find whether the reaction is endothermic or exothermic in nature. by subtracting the initial temperature value with the final temperature one can find the temperature changes.

various combinations of liquid and additive can be used. liquid in this case can be a known quantity of water and additives can be epsom salt or without epsom salt the experiment can be conducted to notice the changes. water and ice, hydrogen peroxide and dry yeast, vinegar and baking soda,etc.