Ergosterol, a precursor of vitamin D, has δmax = 282 nm and molar absorptivity ε = 11,900 M-1cm-1. What is the concentration of ergosterol in a solution whose absorbance A = 0.93 with a sample pathlength l = 1.00 cm. M. What is the absorbance of a solution having a concentration of ergosterol of 4.1×10-5 M with a sample pathlength l = 1.00 cm?

1) is the concentration of ergosterol in a solution.

2) The absorbance of the solution is 0.49.

Explanation:

Using Beer-Lambert's law :

Formula used :

where,

A = absorbance of solution

C = concentration of solution =

l = path length = 1.00 cm

= molar absorptivity coefficient

1)

We have:

Absorbance of the solution ,A = 0.93

Concentration of ergosterol ,C = ?

Path length ,l = 1.00 cm

is the concentration of ergosterol in a solution.

2)

We have:

Absorbance of the solution ,A = ?

Concentration of ergosterol ,C =

Path length ,l = 1.00 cm

The absorbance of the solution is 0.49.