The molar extinction coefficient of cytochrome P450, one of the compounds involved in photosynthesis, at 522 nm is 291 L mol-1cm-1. When light of that wavelength passes through a cell of length 6.5 mm containing a solution of P450, 39.8 % of the light was absorbed. What is the molar concentration of the solution?

0.001165 mol/L is the molar concentration of the solution.

Explanation:

Using Beer-Lambert's law :

Formula used :

where,

A = absorbance of solution

C = concentration of solution

l = path length

T = Transmittance

= incident light

= transmitted light

= molar absorptivity coefficient

We have :

Transmittance of the light  = 100% - 39.8 % = 60.2 % = 0.602

Absorbance of the light :

Molar absorptivity coefficient  =

Length of the cell = l  6.5 mm = 0.65 cm ( 1 mm = 0.1 cm)

Now put all the given values in the above formula, we get the concentration of solution :

0.001165 mol/L is the molar concentration of the solution.

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