To determine the isoelectric point of a protein, first establish that a gel: a) contains a denaturing detergent that can distribute uniform negative charges over the protein surface. b) exhibits a stable ph gradient when ampholytes become distributed in an electric field. c) is washed with an antibody specific to the protein of interest. d) neutralizes all ionic groups on a protein by titrating them with strong bases. e) relates the unknown protein to a series of protein markers with known molecular weights.

The correct answer is B.

Explanation:

To determine the isoelectric point of a protein, first establish that a gel exhibits a stable pH gradient when ampholytes are distributed in an electric field. The isoelectric point is the pH at which a polyampholyte has a net charge of zero. At this pH value, the solubility of the substance is almost zero. Thus, the gel must show a stable pH gradient.

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the density of gold = 19.3 g/cm3

the relation between density and mass and volume is

density = mass / volume

given :

mass of gold = 13 g

density = 13g / volume = 19.3

volume = 13 /19.3 = 0.67 cm^3

thus volume of gold nugget = 0.67 cm^3