The temperature program for a separation starts at a temperature of 50°c and ramps the temperature up to 270°c at a rate of 10°c/minute. which statement is not true for this separation? a) at 10°c/minute, a total of 22 minutes is needed to reach 270°c. b) strongly retained solutes will remain at the head of the column while the temperature is low. c) weakly retained solutes will separate and elute early in the separation. d) the vapor pressure of strongly retained solutes will increase as temperature increases. e) strongly retained analytes will give broad peaks.

the answer is option a.

it can be observed without changing the identity of the object.

it depends on what substance you're talking about as well as many other factors. in quantum mechanics, the observation of particles affects the observers ability to measure certain properties. for example: you can know the velocity of a particle, but you can't know it's position; or you can know it's position, but not it's velocity. it's like trying to find out where a grain of sand is by throwing other grains of sand at it. once you find out where it is, it's been moved. it's also like trying to find an answer to the question of whether or not a tree falling in a forest makes a sound if nobody is there to hear it. paradoxically, the only way to find out is to be there and hear it.