Hexokinase catalyzes the first step of glycolysis, in which glucose is phosphorylated to form glucose-6-phosphate. which of the following statements are accurate? choose all that apply. hexokinase is found in the mitochondrial membrane. hexokinase consists of two domains, or lobes, that come together when glucose and the mgatp2� complex are bound. most kinases require the presence of a monovalent metal ion cofactor to prevent atp hydrolysis. the conformational shift that occurs when glucose, but not water, enters the active site prevents water from hydrolyzing atp. hexokinase transfers the terminal phosphate of atp to carbon 3 of glucose. hexokinase is a type of transferase that catalyzes the transfer of a phosphoryl group from atp to a hexose.