Which of the following statements regarding anfinsen's denaturing experiments with ribonuclease a (rnase a) are valid? exposing the denatured protein to air oxidation and then dialysis to remove urea restored the protein to its original functionality. removing urea by dialysis and then allowing air oxidation of the denatured protein restored the protein to its original functionality. denaturing the protein with both urea and β-mercaptoethanol yielded an inactive protein. anfinsen concluded that protein folding is determined by its primary sequence.

B, C, and D

Explanation:

Anfinsen found that denaturing the protein with both urea and β-mercaptoethanol yielded an inactive protein.  

Removing urea by dialysis and then allowing air oxidation of the denatured protein restored the protein to its original functionality.

He concluded that protein folding is determined by its primary sequence.

A is false. Anfinsen removed the urea first and then allowed the enzyme solution to re-oxidize slowly in air.

a mercury barometer is a device that uses the physical properties of mercury in a tube to measure barometric pressure. mercury barometers are used for : -

1. measurement of atmospheric pressure  

2. determining the altitude of a place

3. weather forecasting

4. calibration and checking of aneroid barometers

5. application in fluid mechanics

6. measurement of pressure in aircrafts

7. surface weather analysis

8. preparation of barographs

9. usage in physical sciences like physics, astronomy & chemistry

10. preparation of aircraft altimeters

characteristics of mercury barometer : -

1.used to define and measure atmospheric pressure.

2.on the average at sea level the column of mercury rises to a height of about 760 mm.

3.this quantity is equal to 1 atmosphere.

4.it is also known as standard atmospheric pressure.

5.a barometer in which the weight of a column of mercury in a glass tube with a sealed top is balanced against that of the atmosphere pressing on an exposed cistern of mercury at the base of the mercury column, the height of the column varying with atmospheric pressure.