Make a 50 ml agarose gel of 0.8% that is 0.5x tbe. the tbe (tris fw = 121.1, borate fw = 61.8, edta fw w = 372) stock is 10x. you have a 150x stock of buffer and you need 2.5 ml of 1x. how do you make this? you have a dna solution that is 21.3 mu g/mu_1 and you want 75 mu 1 that is 50 mu g/mu 1. how do you make this? make 350 ml of a solution that is 25 mm tris, 2.5 mm edta, and the ph is 8.0. tris is a base and the fw of tris is 121.1 and edta is 187. do not make a volume that exceeds 350
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How do temperature and salinity affect deepwater currents? as temperatures and salinity levels of water increase, the water rises to the surface where it creates currents as it moves to colder regions. they create changes in wind direction, moving denser water in the same direction as the wind and causing the deepwater circulation patterns found in the ocean. they equalize the forces on undersea currents caused by the coriolis effect as they replace more dense water with less dense water. they create density differences that cause dense deepwater currents to flow toward the equator where they displace less dense, warmer water above them.
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Make a 50 ml agarose gel of 0.8% that is 0.5x tbe. the tbe (tris fw = 121.1, borate fw = 61.8, edta...
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