Be sure to answer all parts. tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (ka = 5.01 × 10−9 and pka = 8.3) (a) calculate the ph of the tris buffer after mixing 10.0 ml of 0.15 m hcl solution with 25.0 ml of 0.10 m tris. (b) this buffer was used to study an enzyme-catalyzed reaction. as a result of the reaction, 0.00015 mol of h+ was produced. what is the ph of the buffer at the end of the reaction? (c) what would be the final ph if no buffer were present?