What is the role of dna polymerase during dna synthesis? what is the role of dna polymerase during dna synthesis? dna polymerase is the enzyme that catalyzes the addition of a nucleotide onto the 3' end of a growing dna strand. dna polymerase provides the free energy to catalyze the endergonic addition of a nucleotide onto the 3' end of a growing dna strand. dna polymerase removes inorganic phosphate from the template str?