To sort out those bacteria that have the vgp gene, scientists first attempt to grow the bacteria both in a medium with ampicillin and in a medium without ampicillin. demonstrate your understanding of this step by sorting the four bacterial classes based on where they will grow. drag the icon for each bacterial class to the appropriate bin.

Bacteria with no plasmid will ONLY grow in medium without ampicillin. This is because bacteria without any plasmid do not have the gene for ampicillin resistance (ampR). Therefore they can only survive and grow in medium without ampicillin.
Bacteria with nonrecombinant plasmid will grow in both media.According to the image, this type of bacteria has the gene for ampicillin resistance (ampR) in their plasmid. Therefore, they are not affected by the presence of ampicillin in their environment and they can grow in both media.
Bacteria with recombinant plasmid but no vgp gene will grow in both media. These bacteria also have the gene for ampicillin resistance in their plasmid, therefore they can survive and grow in both media.
Bacteria with recombinant plasmid with vgp gene will grow in both media. These bacteria, apart from having the vgp gene in their plasmid, they also have the gene for ampicillin resistance. The vgp gene does not affect the expression of the ampR gene, therefore these bacteria are also ampicillin resistant. 

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