Biology

Biology, 31.07.2019 14:30, bee3176

Give three situations where the negative staining procedure would be used.

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Biology, 21.06.2019 20:00, skylex

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip.nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule.true/false

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Business, 04.10.2019 18:20, 1109pacey

Amicrobiology instructor prepares cultures for a gram-staining laboratory by inoculating growth medium with a gram-positive coccus (non motile) and a gram-negative rod (motile). the goal is to demonstrate staining of a mixed culture of gram negative and gram positive cells. the flask in incubated at 35 c for 24 hours without aeration or shaking. a sample is stained and reveals only gram-negative rods. both cultures are known facultative anaerobes. give a likely reason for success of the gram-negative rod. assume that the cultures have comparable intrinsic growth rates.

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Biology, 26.10.2019 02:43, ehaynie

Give three situations where the negative staining procedure would be used.

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