Atemplate strand of dna is read in the direction in order to direct synthesis of rna in the direction.

The RNA synthesis or ADN transcription occurs in the nucleus and requires:
  - DNA strand to act as a template.
  - Enzymes, specially the RNA-polymerase.
  - Ribonucleotides of Adenine, Guanine, Cytosine and Uracil.
This consist of three maturation of the 3'-->5' direction while the sense of RNA synthesis is 5'-->3'.
This is fulfilled because the enzyme selects the ribonucleotide whose base is complementary to the DNA chain that acts as a template, and binds it by means of a nucleotide bond, releasing a phosphate group.

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