Biology

Biology, 03.08.2019 11:30, YEOng

If 6 molecules of a particular protein were present in an e. coli cell, what would be the nanomolar concentration of this protein?

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Chemistry, 23.06.2019 07:30, shealynh52

Achemist at a pharmaceutical company is measuring equilibrium constants for reactions in which drug candidate molecules bind to a protein involved in cancer. the drug molecules bind the protein in a 1: 1 ratio to form a drug-protein complex. the protein concentration in aqueous solution at 25 ˚c is 1.74 x10-6 m . drug a is introduced into the protein solution at an initial concentration of 2.00 x10-6m. drug b is introduced into a separate, identical protein solution at an initial concentration of 2.00 x10-6m. at equilibrium, the drug a-protein solution has an a-protein complex concentration of 1.00 x10-6m, and the drug b solution has a b-protein complex concentration of 1.40 x10-6m.a. calculate the kc value for the a-protein binding reaction.b. calculate the kc value for the b-protein binding reaction.c. assuming that the drug that binds more strongly will be more effective, which drug is the better choice for further research?

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Chemistry, 10.07.2019 16:30, jgunt8277

Protein a binds to protein b to form a complex, ab: a + b  ab a cell contains an equilibrium mix of protein a at a concentration of 1 µm, protein b at a concentration of 1µm, and the complex ab also at 1 µm. a. what is the equilibrium constant, keq, for the reaction a + b  ab? b. what would the equilibrium constant be if a, b, and ab were each present in equilibrium at a concentration of 1 nm? c. at this lower concentration, about how many extra hydrogen bonds would be needed to hold a and b together tightly enough to form the same proportion of the ab complex? note: free energy change is related to the equilibrium constant by the equation ∆go = -2.3 rt log k, where r is 1.98 x 10-3 kcal/k mole) and t is 310 k.

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Biology, 13.09.2019 19:10, melvindiana58

An investigator purified a protein (protein x) from e.coli. sheinjected protein x into rabbits to generate antibodies thatrecognize and bind to protein x. using an electrophoretictechnique, she separated the proteins from a crude cell extract andused the antibody to locate protein x on the gel. to her surprise,the antibody bound not only with protein x but also with a secondunrelated protein (protein y). when proteins x and y weresequenced, she found that the sequence of residues 67-78, inprotein x and that of residues 120-131 in protein y were identical. the investigator rationalize the data, recognizing thatantigenic determinants (epitopes) of proteins are clusters of aminoacids.

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Chemistry, 25.09.2019 02:10, geckos3411

Aspherical intervertebral disc cell has an outer diameter of 20 um with a nuclear diameter (sphere) of 4 um. protein x is present at a concentration of 35 mg/ml in its cytoplasm. there are some organelles in the intervertebral disc cell that have a cumulative volume of 0.15 picoliters. determine the number of protein molecules within the cell if the molecular weight of the protein is 150,000 daltons (g/mol). (note: avogadro's number is 6.022 x 1023 molecules/mol).

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