You are trying to restriction-map a plasmid. An EcoRI digest gives you a 10 kb fragment, an XhoI digest gives you a 2 kb and an 8 kb fragment, and the double digest gives you fragments of 2, 3, and 5 kb. From these results, you deduce that the size of the plasmid is kb, the number of EcoRI sites is , the number of XhoI sites is , and that there is an EcoRI site within the kb XhoI fragment

-hails tea

explanation:

-t poses

c should be the answer

c. establishing proper sleep hygiene

answer-

the statement that f(x) = |x+a| + b has exactly one x-intercept is sometimes correct.

solution-

it solely depends on b, whether the function will have one or two or zero x intercept.

this plot of the given function, f(x) = |x+a| + b will be the basic absolute value graph i.e v shape, with vertex translated to (-a, b), instead of origin.

1-   if b is zero, the graph will have exactly one x-intercept, at x= -a

2- if b is positive, the whole graph will be above the x-axis, hence it will have no x-intercepts.

3- if b is negative, the graph will be below the x-axis, hence it will have two x-intercepts.

∴ the statement is sometimes true.