![Biology](/tpl/images/cats/biologiya.png)
Biology, 25.11.2021 07:10, Roshaan8039
You identify a mutant that blocks melanosome movement. You rule out any defects in melanosome formation, dynein function or cell signaling. You wonder whether kinesin-2 or the cytoskeleton itself could be disrupted. What is the best way you could determine if and how the subcellular organization of the cytoskeleton is specifically affected for kinesin-2 function in the mutant cells?
a. Perform cell fractionation and then use immunoblots with an antibody against alpha-tubulin to detect microtubules, gamma-tubulin to see centrosomes, and +TIP proteins to see plus (+) ends of microtubules.
b. Perform immunofluorescence with antibodies against alpha-tubulin to detect microtubules, gamma-tubulin to see centrosomes, and + TIP proteins to see plus (+) ends of microtubules.
c. Perform a bead assay, using bead coated with kinesin-2 on slides coated with cell lysates from either wildtype or mutant cells.
d. Perform immunofluorescence to detect F-actin and antibodies to specific capping proteins to detect plus (+) and minus (-) ends of microfilaments.
e. Perform a FRAP assay of fluorescently-labeled microtubules.
![answer](/tpl/images/cats/otvet.png)
Answers: 2
Other questions on the subject: Biology
![image](/tpl/images/cats/biologiya.png)
![image](/tpl/images/cats/biologiya.png)
Biology, 22.06.2019 15:30, gracebuffum
The hardy-weinberg principle is that genetic frequencies in a population? will remain stable from generation to generation will always be changing will not be affected by environmental changes are always in a 3: 1 ratio
Answers: 3
![image](/tpl/images/cats/biologiya.png)
![image](/tpl/images/cats/biologiya.png)
Biology, 22.06.2019 17:30, jamieric0324
Which of the following is not a technology that can be used to conserve resources? a. hydropower b. volcanic power c. natural gas d. geothermal select the best answer from the choices provided a b c d
Answers: 3
Do you know the correct answer?
You identify a mutant that blocks melanosome movement. You rule out any defects in melanosome format...
Questions in other subjects:
![Konu](/tpl/images/cats/en.png)
![Konu](/tpl/images/cats/fizika.png)
![Konu](/tpl/images/cats/en.png)
![Konu](/tpl/images/cats/biologiya.png)
Biology, 12.02.2020 21:25
![Konu](/tpl/images/cats/mat.png)
Mathematics, 12.02.2020 21:25
![Konu](/tpl/images/cats/pravo.png)
![Konu](/tpl/images/cats/mat.png)
Mathematics, 12.02.2020 21:26
![Konu](/tpl/images/cats/istoriya.png)
![Konu](/tpl/images/cats/istoriya.png)