TEST I.
Hela cells (a human cervix carcinoma cell line) were cultured under optimal
conditio...
TEST I.
Hela cells (a human cervix carcinoma cell line) were cultured under optimal
conditions and then left untreated (control) or treated with taxol for the time periods indicated
in the charts. The cells were stained with a fluorescent DNA dye and then subjected to flow
cytometric analysis (Figure 1.).
Review the principle of flow cytometry, study the charts and solve the following
multiple-choice questions!
Control
Tano
Tas
hr
JU
Ta
22 h
Ohr
Cellular DNA content
Figure 1. The effect of taxol treatment on Hela cell cultures (details in the text)
Where does intensive DNA synthesis take place ?
Answers: 1
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