Biology, 26.10.2021 14:00, IDespretlyneedhelp
You have a series of protein solutions of unknown concentration that you saved during your purification procedure. You prepare assay solutions using 50 uL from each of these samples, which yield the follow A595 values:
Sample 1: Crude homogenate: 2.031
Sample 2: Metal ion affinity chromatography fraction: 0.640
Sample 3: G-25 buffer exchange fraction: 0.592
a. Which of these sample concentrations can be reliably determined using your standard curve?
1. 0.086=.0369x+.0495
x=.98915
2. 0.13=.0369x+.0495
x= 2.18157
3. 0.037=.0369x+.0495
x= -.33875
4. 0.009=.0369x+.0495
x=-1.09756
5. 0.011=.0369x+.0495
x=-1.04336
6. 0.008=.0369x+.0495
x=-1.12466
b. What is the protein concentration in these samples (in mg/mL)?
c. How would you measure protein concentrations for the sample(s) whose concentrations cannot be reliably determined from the values given?
d. Is the Bradford protein assay the best choice for this analysis? Why or why not?
e. Suppose the molecular weight of your protein of interest is 32,500 daltons. What is the molar concentration of protein in the final purified protein in the G-25 buffer exchange fraction?
Answers: 2
Biology, 22.06.2019 01:00, suselygonza
Which of the following would be a body response when stepping outside without a coat on a snowy day? a. senaceous glands. secrete more senum b. more liquid is transported by the body to the skin fir insulation c. blood vessels in the skin narrow and contract d. blood vessels in the skin expand
Answers: 1
You have a series of protein solutions of unknown concentration that you saved during your purificat...
Mathematics, 09.03.2021 22:30
History, 09.03.2021 22:30
Social Studies, 09.03.2021 22:30
Mathematics, 09.03.2021 22:30