5. You are working to determine the TCID50 of an unknown virus which you infected onto MDCK cells. The following table are your results: Virus Dilution Positive Wells Negative Wells Total Wells % infection
10^-1 24 0 24 100
10^-2 24 0 24 100
10-3 16 1 17 94
10-4 9 4 13 69
10-5 4 9 13 31
10-6 1 16 17 6
10-7 0 24 24 0

Using this data, which of the following is correct for the estimated TCID50:
a. The TCID50 is 10^-3.5
b. The TCID50 is 10^-4.5
c. The TCID50 is 10^-3.25
d. The TCID50 is 10^-4.25
e. The TCID50 is 10^3.25
f. The TCID50 is 10^4.25

Which layer will become saturated first when rain falls?

Bisphenol a (often called bpa) is a chemical found in products that people use every day, from water bottles to food containers to children's toys. unfortunately, bpa leaches out of its many products and makes its way into our bodies. what are the health effects of bpa exposure? ongoing research is finding that elevated exposure to bpa can affect a wide variety of developmental and physiological processes, but one of the first studies of bpa's health effects came about because of a simple mistake in the lab. at a laboratory at case western reserve university in 1998, geneticist patricia hunt was making a routine check of her female lab mice. as she extracted and examined developing eggs from the ovaries, she began to wonder what had gone wrong. she noticed that many of the eggs showed problems with their chromosomes, and some had irregular amounts of genetic material, which can lead to miscarriages and birth defects in mammals. she learned that a lab assistant had mistakenly washed the plastic mouse cages and water bottles with a harsh soap, releasing bpa from the plastic. knowing that bpa is an endocrine disruptor, a chemical that can enter organisms and mimic hormones, hunt set out to discover whether it had adversely affected her mice.

10. in which phase will crossing-over occur? a. mitosis b. metaphase ii c. prophase i d. meiosis ii