State briefly the general principles of classification of living organisms

Recent advances in biochemical and electron microscopic techniques, as well as in testing that investigates the genetic relatedness among species, have redefined previously established taxonomic relationships and have fortified support for a five-kingdom classification of living organisms. This alternative scheme is presented below and is used in the major biological articles. In it, the prokaryotic Monera continue to comprise the bacteria, although techniques in genetic homology have defined a new group of bacteria, the Archaebacteria, that some biologists believe may be as different from bacteria as bacteria are from other eukaryotic organisms. The eukaryotic kingdoms now include the Plantae, Animalia, Protista, and Fungi, or Mycota.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.