What are the features of CRISPRa Stable Cell Lines? CRISPRa (CRISPR activation) system is a technology based on the improved CRISPR/Cas9 system that uses a synthetic programmable transcription factor to initiate endogenous transactivation of genes. In mammalian cells, the expressing of dCas9 (a nuclease-deficient Cas9 only retaining DNA binding activity) fused with a transcriptional activation domain, and gRNA complementary to the target promoter region in the genome, will result in increased transcription of the corresponding gene.

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