Enzymes can be denatured if: * .not enough substrate is present.
. too many enzymes are present.
.the temperature or pH is too high or too low.
.the temperature and pH are within normal ranges.

Ihaven't done that yet, so no idea,but this is not the right place to ask

Rnabothrnabothdnadnaboth

Are you asking about a street map usage  if so here is your answer  a street map for a very limited area could simply be a bit map or a .jpg image.  but a more general map would probably store the streets as vectors.  but you shouldn't assume that there is one way to do it