5-In the enzyme chymotrypsin, aspartate 102 is relatively far from the substrate; Asp 102 helps position His 57, and raises the pKa of His 57 to allow the histidine to act as a base. This is important to the catalytic process and the mutation of Asp 102 to asparagine decreases the Kcat by ~10,000-fold. The wild-type enzyme k cat was 100 s-1. What amount of binding energy does this represent