Students have been using a cell-free system that used room-temperature stable protein of interest. To stop the reaction, they use heat to the proteins to high temp then add negatively charged detergents. This allows the students to separate the enzymes, the protein targets, and their fragments on an electrophoresis gel. What happens to enzymes and other proteins when they are heated up and soaked in detergent? How would the larger enzymes separate from the protein fragments on gel? How would a protein that is cut look before and after the experiment? Describe the mechanism for gel electrophoresis.

you are certainly talking about this picture:

a: is the label of the white square, it is showing the  replication fork, formed by helicase (d) ==> so it is the site of dna separation and replication 

b: is a new dna brand that is following directly the replication fork, so it is continuous ==> built continuously from 5' to 3

c: dna polymerase cannot replicate dna on its own, it needs the of their proteins, and one of them plays the role of a clamp which fixes  the dna with the dna template and increases  the processivity of the enzyme. ==>   stops polymerase from falling off the  template.

d: is the enzyme responsible for forming the replication fork (helicase) ==> unwinds and separates dna into two strands

e: is the delayed strand, it is built  in the opposite sense of the helicase, so dna will be built in fragments ==> built  fragments from 5' to 3 joins

and finally f: is the enzyme that builds  the new dna strand (dna polymerase) ==>   adds nucleotides to make a new dna helix.

credit: gradman555p98x9p

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weathering - colorado river carves land in arizona to form the grand canyon. cracks form in a sandstone wall surrounding a house. a marble statue near the science museum is changing color due to rainfall

erosion - after a rainstorm, soil and rock piees litter the roadways. the sand dunes in the sahara desert are changing shape.

here's your answer.

estuaries are the partially enclosed coastal bodies of brackish water formed at the mouth of sea where river meets an ocean. it is a transition zone between river and sea water so estuaries are influenced to both marine environment such as tides, waves and to riverine such as flow of fresh water and sediments. the mixing of sea and fresh water provide high level of nutrients, making estuaries a high product habitats.

many estuaries suffers degradation from a variety of human activities like erosion from deforestation, overgrazing, overfishing, drainage and eutrophication.

so from the above statement answers will be

1. false as estuaries are formed due to both brackish and freshwater.

2.true, due to high productivity estuaries are breeding ground for many marine species.

3. direct and indirect activities of man that contribute to pollution and deposition of sediments.